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bsbdenovo-datasets [2017/11/14 04:40]
telatina
bsbdenovo-datasets [2017/11/14 07:36] (current)
telatina
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 </​code>​ </​code>​
 This phage is apparently 114,180 bp long, and of course the reference only has 1 sequence in total. This phage is apparently 114,180 bp long, and of course the reference only has 1 sequence in total.
 +
 +==== QC [Optional] ====
 +Since we already managed to try the FastQC program to summarize some quality metrics about NGS data, we can skip the section. If you are interested you can have a look at the output **[[http://​147.188.173.45/​public/​researcher/​bsb07/​QC|clicking here]]**.
 +
 +Let's create a directory where to save the output of FastQC:
 +<code bash>
 +mkdir ~/web/QC
 +</​code>​
 +
 +In your home directory there is a ''​~/​web/''​ directory that can be accessed from the web. That's why we created a ''​QC''​ directory inside it.
 +
 +Now let's perform the QC:
 +
 +<code bash>
 +fastqc -o ~/web/QC /​bsb/​denovo/​phage/​reads/​shotgun*.fq
 +</​code>​
 +
 +At the end visit [[http://​147.188.173.45/​public/​researcher/​|this page]], select your username and see the output.